Antibacterial Performance of a Cu-bearing Stainless Steel against Microorganisms in Tap Water

This document is the Accepted Manuscript of the following article: Mingjun Li, Li Nan, Dake xu, Guogang Ren, Ke Yang, ‘Antibacterial Performance of a Cu-bearing Stainless Steel against Microorganisms in Tap Water’, Journal of Materials Science & Technology, Vol. 31 (3): 243-251, March 2015, DOI: https://doi.org/10.1016/j.jmst.2014.11.016, made available under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License CC BY NC-ND 4.0 (http://creativecommons.org/licenses/by-nc-nd/4.0/).Tap water is one of the most commonly used water resources in our daily life. However, the increasing water contamination and the health risk caused by pathogenic bacteria, such as Staphylococcus aureus and Escherichia coli have attracted more attention. The mutualism of different pathogenic bacteria may diminish antibacterial effect of antibacterial agents. It was found that materials used for making pipe and tap played one of the most important roles in promoting bacterial growth. This paper is to report the performance of an innovative type 304 Cu-bearing stainless steel (304CuSS) against microbes in tap water. The investigation methodologies involved were means of heterotrophic plate count, contact angle measurements, scanning electron microscopy for observing the cell and subtract surface morphology, atomic absorption spectrometry for copper ions release study, and confocal laser scanning microscopy used for examining live/dead bacteria on normal 304 stainless steel and 304CuSS. It was found that the surface free energy varied after being immersed in tap water with polar component and Cu ions release. The results showed 304CuSS could effectively kill most of the planktonic bacteria (max 95.9% antibacterial rate), and consequently inhibit bacterial biofilms formation on the surface, contributing to the reduction of pathogenic risk to the surrounding environments.Peer reviewe

The Danger Is Growing! A New Paradigm for Immune System Activation and Peripheral Tolerance

Successful immune defense is a complex balancing act. In order to protect a host against invasion by harmful pathogens, an immune response must be rapid and vigorous, and must eliminate foreign invaders before their populations grow beyond control. That same immune response, however, must be selective enough to recognize and ignore commensal bacteria, environmental antigens and host tissue itself. How the immune system makes the crucial decision whether or not to attack a particular antigen has been a long-standing question central to the study of immunology. Here we show that the structure of the signaling network between regulatory T-cells and type 17 helper T-cells allows the immune system to selectively attack pathogens based on whether or not the pathogens represent a growing, and thus dangerous population. We term this mechanism for immune system activation the ‘Growth Detection Paradigm’, because it offers an entirely new explanation for immune system regulation and peripheral tolerance

A web-DB model on multicast and anycast

Most of the current web-based database systems suffer from poor performance, complicated heterogeneity, and synchronization issues. In this paper, we propose a novel mechanism for web-based database system on multicast and anycast protocols to deal with these issues. In the model, we put a castway, a network interface for database server, between database server and Web server. Castway deals with the multicast and anycast requests and responses. We propose a requirement-based server selection algorithm and an atomic multicast update algorithm for data queries and synchronizations. The model is independent from the Internet environment, it can synchronise the databases efficiently and automatically. Furthermore, the model can reduce the possibility of transaction deadlocks.<br /

A natural mutation-led truncation in one of the two aluminum-activated malate transporter-like genes at the Ma locus is associated with low fruit acidity in apple

Acidity levels greatly affect the taste and flavor of fruit, and consequently its market value. In mature apple fruit, malic acid is the predominant organic acid. Several studies have confirmed that the major quantitative trait locus Ma largely controls the variation of fruit acidity levels. The Ma locus has recently been defined in a region of 150kb that contains 44 predicted genes on chromosome 16 in the Golden Delicious genome. In this study, we identified two aluminum-activated malate transporter-like genes, designated Ma1 and Ma2, as strong candidates of Ma by narrowing down the Ma locus to 65-82kb containing 12-19 predicted genes depending on the haplotypes. The Ma haplotypes were determined by sequencing two bacterial artificial chromosome clones from G.41 (an apple rootstock of genotype Mama) that cover the two distinct haplotypes at the Ma locus. Gene expression profiling in 18 apple germplasm accessions suggested that Ma1 is the major determinant at the Ma locus controlling fruit acidity as Ma1 is expressed at a much higher level than Ma2 and the Ma1 expression is significantly correlated with fruit titratable acidity (R 2=0.4543, P=0.0021). In the coding sequences of low acidity alleles of Ma1 and Ma2, sequence variations at the amino acid level between Golden Delicious and G.41 were not detected. But the alleles for high acidity vary considerably between the two genotypes. The low acidity allele of Ma1, Ma1-1455A, is mainly characterized by a mutation at base 1455 in the open reading frame. The mutation leads to a premature stop codon that truncates the carboxyl terminus of Ma1-1455A by 84 amino acids compared with Ma1-1455G. A survey of 29 apple germplasm accessions using marker CAPS1455 that targets the SNP1455 in Ma1 showed that the CAPS1455A allele was associated completely with high pH and highly with low titratable acidity, suggesting that the natural mutation-led truncation is most likely responsible for the abolished function of Ma for low pH or high acidity in appl

Incident laser modulation by tool marks on micro-milled KDP crystal surface: Numerical simulation and experimental verification

© 2019 Elsevier Ltd Micro-milling has been accepted as the most promising method to repair the micro-defects on the surface of KH2PO4 (KDP) optics. However, surface tool marks are inevitably introduced during the micro-milling repairing process, and could possess great potential risks in lowering the laser-induced damage threshold of KDP optics. The primary cause of laser damage growth of nonlinear crystals has been considered as its internal light intensification. In this work, how the tool marks impact the incident laser modulation as well as the laser-induced damage resistance of micro-milled KDP optics was theoretically and experimentally investigated. The results indicate that periodic tool marks can cause diffraction effect and result in significant relative light intensity modulation (IRmax), up to 5.6 times higher than that inside smooth crystal surfaces. Although the change trends of IRmax with respect to tool marks on both surfaces of KDP optics are similar, the IRmax induced by the rear-surface tool marks is nearly twice higher than that induced by the front-surface tool marks, which means the rear surface with tool marks are more vulnerable to be damaged. The period of tool marks determines the modulation degree and distribution patterns of light intensity inside KDP crystal while the residual height of tool marks can only slightly regulate the modulation degree of light intensity. The tool marks with a period of 1 μm normally give rise to serious light intensification and should be strictly excluded, while the period of tool marks from 10 μm to 20 μm is conducive to the laser damage resistance of micro-milled KDP optics, which were verified by the tests of transmittance capacity and laser damage resistance, and is supposed to be preferred in the actual repairing process of full-aperture KDP optics

Spontaneous electric-polarization topology in confined ferroelectric nematics

Topological spin and polar textures have fascinated people in different areas of physics and technologies. However, the observations are limited in magnetic and solid-state ferroelectric systems. Ferroelectric nematic is the first liquid-state ferroelectric that would carry many possibilities of spatially distributed polarization fields. Contrary to traditional magnetic or crystalline systems, anisotropic liquid crystal interactions can compete with the polarization counterparts, thereby setting a challenge in understating their interplays and the resultant topologies. Here, we discover chiral polarization meron-like structures during the emergence and growth of quasi-2D ferroelectric nematic domains, which are visualized by fluorescence confocal polarizing microscopy and second harmonic generation microscopies. Such micrometre-scale polarization textures are the modified electric variants of the magnetic merons. Unlike the conventional liquid crystal textures driven solely by the elasticity, the polarization field puts additional topological constraints, e.g., head-to-tail asymmetry, to the systems and results in a variety of previously unidentified polar topological patterns. The chirality can emerge spontaneously in polar textures and can be additionally biased by introducing chiral dopants. An extended mean-field modelling for the ferroelectric nematics reveals that the polarization strength of systems plays a dedicated role in determining polarization topology, providing a guide for exploring diverse polar textures in strongly-polarized liquid crystals